磷酸饥饿时番茄幼苗酸性磷酸酶活性的变化与Pi吸收的关系

Plant Diversity ›› 1999, Vol. 21 ›› Issue (01): 1-3.

• 研究论文 •

磷酸饥饿时番茄幼苗酸性磷酸酶活性的变化与Pi吸收的关系

宋克敏,焦新之,李琳,颜季琼

华中农业大学农学系

出版日期:1999-02-25 发布日期:1999-02-25

Relationship between the Changes of Acid Phosphatase Activities and Pi -uptake of Tomato Seedlings during Phosphate Starvation

SONG Ke -Min, JIAO Xin -Zhi, LI Lin, YAN Ji -Qiong

Department of Agronomy, Huazhong Agricultural University, Wuhan 430070

Online:1999-02-25 Published:1999-02-25

摘要/Abstract

摘要： 磷酸饥饿时，番茄幼苗根部及地上部酸性磷酸酶活性均显著增强，根部细胞表面酸性磷酸酶及根部外泌的酸性磷酸酶活性亦明显提高。动力学分析表明，磷酸饥饿提高了番茄幼苗根部的酸性磷酸酶对其底物的亲和力。另外，磷酸饥饿对番茄幼苗根部酸性磷酸酶活性的最适pH值没有影响。钥酸对番茄幼苗根部酸性磷酸酶活性有强烈的抑制作用，对番茄幼苗Pi吸收速率也有十分明显的抑制效果。以上结果表明，磷酸饥饿时，番茄幼苗Pi吸收的适应性变化可能与根部酸性磷酸酶特别是根部细胞表面酸性磷酸酶及其外泌酸性磷酸酶的参与密切关联。

关键词: 番茄, 磷酸饥饿, 酸性磷酸酶, 磷吸收

Abstract: The acid phosphatase activities from roots and both stems and leaves of tomato seedlings all in-creased markedly under phosphate starvation. Phosphate starvation also increased the activities of acid phos-phatase from cell surface of, and released by roots of tomato seedlings. The kinetic analysis of acid phos-phatase of roots of tomato seedlings revealed that phosphate starvation increased the affinity of the enzyme to its substrate. The results also revealed that phosphate starvation had no effect on the optimum pH (pH 4.93) of the acid phosphatase of roots of tomato seedlings. It was also found that molybdate strongly inhibited not only the activities of acid phosphatase but also Pi- uptake rates of tomato seedlings.

Key words: Tomato (Lycopersicon esculentum)

宋克敏,焦新之,李琳,颜季琼. 磷酸饥饿时番茄幼苗酸性磷酸酶活性的变化与Pi吸收的关系[J]. Plant Diversity, 1999, 21(01): 1-3.

SONG Ke -Min, JIAO Xin -Zhi, LI Lin, YAN Ji -Qiong. Relationship between the Changes of Acid Phosphatase Activities and Pi -uptake of Tomato Seedlings during Phosphate Starvation[J]. Plant Diversity, 1999, 21(01): 1-3.

[1]	李婉莎, 刘德团, 杨永平, 胡向阳. 番茄茎腺毛差异表达序列分离与分析[J]. Plant Diversity, 2011, 33(6): 660-666.
[2]	段继强1 , 2 , 李建永1 , 吴丽艳1 , 梁雪妮1 , 刘飞虎1 . 苎麻细胞质雄性不育“三系”ISSR 特异片段克隆和序列分析[J]. Plant Diversity, 2008, 30(6): 706-712.
[3]	伊淑莹, 孙爱清, 赵春梅, 刘箭. 番茄多胁迫诱导型LeMTshsp 启动子的分子克隆及其功能分析[J]. Plant Diversity, 2007, 29(02): 223-230.
[4]	何培青，张鹏英，陈靠山. 番茄几种挥发性组分对番茄灰葡萄孢的抑制作用[J]. Plant Diversity, 2005, 27(03): 315-320.
[5]	沈文涛，周鹏. 轮状病毒外壳蛋白VP7在转基因番茄果实中的特异表达[J]. Plant Diversity, 2004, 26(02): 1-3.
[6]	江晓玲俞守义贺竹梅彭志强祁瑜. 番茄子叶外植体转化系统的优化[J]. Plant Diversity, 2004, 26(01): 1-3.
[7]	宋克敏　焦新之　李琳等　　. 磷酸盐饥饿时番茄幼苗根部质膜蛋白组分的变化[J]. Plant Diversity, 2000, 22(03): 1-3.
[8]	范黎,郭顺星,肖培根. 墨兰菌根的结构及酸性磷酸酶定位研究[J]. Plant Diversity, 1999, 21(02): 1-3.
[9]	宋克敏；焦新之；李琳；颜季琼. 磷酸饥饿对番茄幼苗生长状况及其磷吸收的影响[J]. Plant Diversity, 1998, 20(03): 1-3.
[10]	刘成运. 云南山茶雄配子体发育过程中三磷酸腺苷酶及酸性磷酸酶细胞化学定位[J]. Plant Diversity, 1984, 6(04): 1-3.

磷酸饥饿时番茄幼苗酸性磷酸酶活性的变化与Pi吸收的关系

Relationship between the Changes of Acid Phosphatase Activities and Pi -uptake of Tomato Seedlings during Phosphate Starvation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价