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Plant Diversity ›› 1999, Vol. 21 ›› Issue (02): 1-3.

• 研究论文 •    

竹柏类植物的RAPD分析

王艇,苏应娟,黄超,朱建明   

  1. 中山大学生命科学学院,广州 510275
  • 出版日期:1999-04-25 发布日期:1999-04-25

RAPD Analyses of Nageiaoids

WANG Ting,SU Sing-Juan,HUANG Chao,ZHU Jian-Ming   

  1. School of Life Sciences, Zhongshan University, Guangzhou 510275
  • Online:1999-04-25 Published:1999-04-25

摘要: 对鸡毛松(Podocarpus imbricatus)、竹柏(Podocarpus nagi)、长叶竹柏(Podocarpus ryi )、百日青(Podocarpus neriifolius)和罗汉松(Podocarpus macrophyllus)进行了 RAPD分析。经筛选4组80个引物,发现9个引物的带型清晰并呈多态性。采用UPGMA法对求出的遗传距离进行聚类分析,得到的结果显示:本研究中的植物明显地分为3个亚类群,支持在罗汉松属内建立竹柏组的处理方式,不同意把竹柏类从罗汉松属中分离出去成立新科的观点。

关键词: 竹柏类, RAPD分析

Abstract: Choosing Podocarpus imocarpus imbricatus,Podocarpus nagi,Podocarpus fleuryi,Podocarpus neriifolius and Podocarpus macrophyllus are analysed through the RAPD techniques.Eighty primers from 4 operon kits were screened and 9 of them were able to produce clear polymorphic patterns.The results generated by using UPGMA algorithm to cluster genetic distances calulated from RAPD data imply: 1)the studied species are apparently clustered into three subgroups,which aupports the treatment of constructing corresponding sections,Sect.Dacrycarpus ,Sect.Nageia and Sect. Podocarpus in the genus Podocarpus,2)it is unreasonable to separate nageiaoids from Podocarpus and to establish a new family named Nageiaceae

Key words: Nageiaoids, RAPD analysese