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Plant Diversity ›› 2003, Vol. 25 ›› Issue (14): 1-3.

• 研究论文 •    

民族生物学学科规范

民族生物学工作小组, 许建初、陈微 译   

  1. 昆明植物研究所
  • 出版日期:2003-12-26 发布日期:2003-12-26

RAPD Analysis on the Genetic Variation of Erigeron breviscapus from Yunnan

ZHOU LiJie,LI NanGao,YU Hong,ZHANG ShiGang   

  1. Laboratory of Ecologica Genetica,College of Life Science,Yunnan University,Kunming 650091,China;Inmol Laboratory of Biotechnology of Yunnan,Kunming 650106,China
  • Online:2003-12-26 Published:2003-12-26

摘要: 民族生物学是研究人(民族)、生物区系和环境之间动态关系的一门学科。民族生物学涉及多个学科领域,包括:系统分类学、种群生物学、生态学、生物数学、文化人类学、民族学、考古学、地理学、药理学、营养学以及保护和可持续发展学。随着民族生物学愈来愈为美国国内外的关注,美国民族植物学工作小组受美国国家科学基金(NSF)的委托,为讨论和制定民族生物学学科规范,召集了国际上活跃的35位民族生物学家于2002年4月4日-6日在密苏里植物园召开了会议。该民族生物学工作组回顾了该学科的一些重要领域,包括研究内容、研究方法、分析手段、教育、以及基金申请等,积极探索适合于研究人-生物-环境三者间互相作用的现代民族生物学方法。工作小组认为对建立在假设上的多学科数据进行定量分析、发展跨学科的教育项目以培养民族生物学的新生力量,获得更广泛的科研经费来源是迫在眉睫的任务。

Abstract: Random amplified polymorphic DNA (RAPD) was amplified to studied genetic diversity and genetic structure of 6 populations of Erigeron breviscapus in Yunnan province.As a contrast,one population of Aster himalaicus was selected to study their phylogenetic relationship.A total of 16 primers were screened to use,and 233 bands were amplified,among which 192 (8240%) were polymorphic.At specific level,the percentage of polymorphic bands PPB,number of alleles A,effective number of alleles Ae,Nei's gene diversity H,and Shannon's Information index I were 8240%,18240,13005,01896,and 03021 respectively,and at population level were on average 5423%,15401,12691,01607,and 02460 respectively.Gst was 03460,meaning that 6540% of the genetic variation was found within the populations.The genetic identity among the populations was high,ranging from 09037 to 09723 and averaging 09410.An analysis of 6 populations using UPGMA showed that,there was the closest relationship between Qiubei (YB) population and Wenshan (YX) population,then between Xiaoshao (Y) and Xiping (YA) populations,and the last between Wenshan (YX) population and Tengchong (ZA) population.The genetic distance is almost positively related to the spatial or geographical distance between the populations.

Key words: Erigeron breviscapus