盾叶薯蓣花药培养及单倍体植株的获得

Plant Diversity ›› 2007, Vol. 29 ›› Issue (01): 33-37.

盾叶薯蓣花药培养及单倍体植株的获得

闫立霞, 胡春根, 姚家玲

华中农业大学生命科学技术学院

收稿日期:2006-03-29 出版日期:2007-02-25 发布日期:2007-02-25
通讯作者: 姚家玲

Haploid Callus and Plantlets Regenerated from Anther Culture of Dioscorea zingiberensis (Dioscoreaceae)

YAN Li-Xia, HU Chun-Gen, YAO Jia-Ling

College of Life Science and Technology , Huazhong Agricultural University

Received:2006-03-29 Online:2007-02-25 Published:2007-02-25
Contact: YAO Jia-Ling

摘要/Abstract

摘要： 以处于单核期的盾叶薯蓣花药为材料, 经愈伤组织途径成功获得单倍体盾叶薯蓣新材料。对盾叶薯蓣花药培养、植株再生过程的研究表明: 来自不同居群的外植体对花药愈伤组织诱导有显著影响; W14 是适合花药愈伤组织诱导的基本培养基; 花药愈伤组织增殖和分化的适宜培养基为: MS 基本培养基+ BA2.0 mg/L + IAA0.2 mg/L; 生根培养基为添加了IBA2.0 mg/L、NAA0.4mg/L 和0.5 g/L 活性碳的MS 培养基。以流式细胞仪和根尖染色体压片法检查花培植株的结果表明, 有8%～12% 的个体为单倍体。实验结果为进一步开展单倍体育种及相关理论研究提供了材料和技术基础。

关键词: 盾叶薯蓣, 花药培养, 单倍体, 植株再生FONT

Abstract: Haploid plantlet and callus were induced from the anther culture of Dioscorea zingiberensis as the explant at the stag of later uninucleate period. The results indicates that the basal culture media and explants from different population affect dramatically callus induction from anthers . W14 was the best basal media for the first step of callus induction. MS media with BA2.0 mg/L + IAA0.2 mg/L improved callus′multiplication and differentiation. MS media with 2.0 mg/L IBA, 0.4 mg/L NAA and 0.5 g/L active carbon were suitable for rooting. Examination of the anther cultured plantlet displayed that 8%- 12% clones were haploid. The established technique system and obtained haploid plant would provide solid support and valuable materials for further breeding of Dioscorea zingiberensis .

Key words: Dioscorea zingiberensis

中图分类号:

Q945

闫立霞, 胡春根, 姚家玲. 盾叶薯蓣花药培养及单倍体植株的获得[J]. Plant Diversity, 2007, 29(01): 33-37.

YAN Li-Xia, HU Chun-Gen, YAO Jia-Ling. Haploid Callus and Plantlets Regenerated from Anther Culture of Dioscorea zingiberensis (Dioscoreaceae)[J]. Plant Diversity, 2007, 29(01): 33-37.

[1]	李焘, 林文媛, 王喆之. 菘蓝花药培养及单倍体的诱导[J]. Plant Diversity, 2011, 33(2): 225-228.
[2]	杭悦宇，黄春洪，穆森，徐珞珊. 盾叶薯蓣叶片形态多样性研究[J]. Plant Diversity, 2004, 26(04): 1-3.
[3]	黄春洪杭悦宇周义锋史德荣郭可跃. 我国盾叶薯蓣居群遗传结构分析[J]. Plant Diversity, 2003, 25(06): 1-3.
[4]	钟华鑫潘向群陈汉民郁昭愈梁海曼. BA田间预处理对大麦花药若干生理性状和培养效率的影响[J]. Plant Diversity, 1992, 14(02): 1-3.
[5]	唐世蓉姜志东 . 盾叶薯蓣地上部分的三个新甾体皂甙 [J]. Plant Diversity, 1987, 9(02): 1-3.
[6]	庄承纪. 单倍体水稻体细胞组织的植株再生及其激素调节[J]. Plant Diversity, 1981, 3(02): 1-3.