用细胞学方法研究番木瓜组培苗的遗传稳定性

Plant Diversity ›› 2006, Vol. 28 ›› Issue (06): 645-648.

用细胞学方法研究番木瓜组培苗的遗传稳定性

李卫东; 王冬梅; 周鹏; 吴廷广

中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室

收稿日期:2006-01-19 出版日期:2006-12-25 发布日期:2006-12-25
通讯作者: 周鹏

Study on the Genetic Stability of Carica papaya (Caricaceae) Propagating Plantlets Through Cytological Approach

LI Wei-Dong; WANG Dong-Mei; ZHOU Peng; WU Ting-Guang

State Key Laboratory of Tropical Crop Biotechnology , Institute of Tropical Biology Sciences

Received:2006-01-19 Online:2006-12-25 Published:2006-12-25
Contact: ZHOU Peng

摘要/Abstract

摘要： 利用细胞学和形态学方法研究组织培养技术生产的番木瓜种苗的遗传稳定性。通过对番木瓜(2n =18) 主栽品种蔬罗1 号1～38 代组培苗体细胞的染色体数的初步观察和统计, 发现1～32 代苗所观察的体细胞染色体全部为2n = 18 , 大田种植后表型与亲代相比未发生明显的变异。但是在第33 , 36 , 37 代发现了染色体非整倍性的细胞, 田间种植后与亲代相比挂果率和抗病性下降。这一研究结果为番木瓜组培苗的继代培养和规模化生产提供了有价值的理论依据。

关键词: 番木瓜组培苗, 染色体非整倍性, 遗传稳定性FONT

Abstract: The genetic stability of papaya propagating plantlets was studied through cytological and morphological approachs. Primary observation and accountment were holded on chromosome numbers of propagating plantlets of papaya , Solo
I , which is major species of Hainan province from1 to 38 generations, the results showed that the numbers of chromosome are all 18 (2n = 18) from 1 to 32 generations . In addition there were no difference compared with their parents when they were planted in fields . The cells which chromosome were non-integral were found in the leaves of 33rd and 36th and 37th generation, and the fructiferous rate and anti-virus character of them were dropped when the plantlets were planted in field. This showed that there was some difference in the level of cell chromosome after cultured continuously 32nd generation. This result could give somevaluable theoretic reference to the tissue culture and industrialized produce of papayapropagating plantlets.

Key words: Carica papaya propagating plantlets

李卫东; 王冬梅; 周鹏; 吴廷广. 用细胞学方法研究番木瓜组培苗的遗传稳定性[J]. Plant Diversity, 2006, 28(06): 645-648.

LI Wei-Dong; WANG Dong-Mei; ZHOU Peng; WU Ting-Guang. Study on the Genetic Stability of Carica papaya (Caricaceae) Propagating Plantlets Through Cytological Approach[J]. Plant Diversity, 2006, 28(06): 645-648.