Abstract: The application of multimedia information system on microbiology research is microbiology resource database constructed by writiongs,graphs,sounds and pictures etc．．The graph and writing database of the two group of Streptomyces and Thermophilic actinomycetes will be estabilished first．This database is consisted of software and results from species classification research,research works,as morphological graph(aerial mucelium,spore,spore ultrathin-section),graphic spectrum(analisis graph spectrum of constituents of wall Chemotypes,segments of DNA and RNA,ultraviolet graph spectrum of metabolite),value(UPGMA analysis of different species),writing(names, characteristics of physiology and biochemistry,culture medium and preservation,habitat products)．The pictures byusing scanner and writing information will be edited and transfered．This resource can be utilized for classification research,resourse inquiry,domestic and international exchange．