Biodiv Sci ›› 1998, Vol. 06 ›› Issue (1): 37-41.  DOI: 10.17520/biods.1998007

• 论文 • Previous Articles     Next Articles

The analysis of genetic relationship among some Allium cultivars determined by RAPD markers

MENG Xiang-Dong,MA Hong,ZHANG Wei-Hua   

  1. Department of Horticulture , Shandong Agricultural University , Taian  271018
  • Received:1996-10-15 Revised:1997-06-20 Online:1998-02-20 Published:1998-02-20

Abstract: Nine cultivars belonging to four species and one hybrid of Allium commonly cultivated in China were analyzed by RAPD markers to determine their genetic relationship. Of the 20 decamer nucleotide primers screened , 11 showed consistent banding patterns and amplification and produced 102 bands with 68 polymorphic. The similar indices among the materials ranged from 0. 571 to 0. 947. A . fistulosum var. gigantum cv. Zhangqiu Welsh onion , A . fistulosum var. gigantum cv. Tianjin Wuyeqi Welsh onion , A . fistulosum var. caepitosum cv. Shanghai Spring onion and A . fistulosum var. caepitosum cv. Nanjing Winter onion (Spring onion) had the highest similar indices of 0. 947 and 0. 942 respectively. Relationship between the cultivars determined by UPGMA cluster analysis demonstrated Zhangqiu Welsh onion , Tianjin Wuyeqi Welsh onion , Shanghai Spring onion and Nanjing Winter onion were clustered together respectively. The two Welsh onion cultivars , two Spring onion cultivars and Welsh onion from Korea were clustered very closely. A . schoenoprasum and A . fistulosum var. viviparum were subclustered further in the group of A . fistulosum , including A . fistulosum , A . schoenoprasum , A . ascalonicum and A . cepa were clustered together. The hybrid of Zhangqiu Welsh onion ×A . cepa was clustered very closely with A . ascalonicum and A . cepa , but clustered with the group of A . fistulosum with the furthest linkage distance. A . ascalonicum and A . cepa could be included in the group of A . cepa. The taxonomy of A llium was discussed on the basis of RAPD markers.