Biodiv Sci

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Culturable bacterial diversity in rhizosphere of Bashania fangiana

Min Liu1,2, Lubin Li2, Kai Yang3, Jigang Han1, Baocheng Zhu1,4*, Zhenhua Peng2   

  1. 1 College of Life Science, Hebei University, Baoding 071002
    2 Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091
    3 Key Laboratory of New Technology in Agricultural Application in Beijing, Beijing Agricultural College, Beijing 102206
    4 College of Life Science, Agricultural University of Hebei, Baoding 071001
  • Received:2007-09-03 Revised:2007-11-15 Online:2008-01-27 Published:2008-01-27
  • Contact: Baocheng Zhu

Abstract:

In order to understand the diversity of culturable bacteria, we collected Bashania fangiana rhizosphere soil in Wolong Nature Reserve in May 2006. Then we isolated and cultured 50 bacteria strains with different morphological character of colonies from the soil. Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the 50 strains were clustered into ten genera: 27 strains of Gammaproteobacteria (42.3%), nine strains of Firmicutes (26.9%), four strains of Actinobacteria (15.4%), six strains of Betaproteobacteria (7.7%), three strains of Alphaproteobacteria (3.9%), one strain closely related to the ge-nus Pedobacter. The dominant bacteria were Pseudomonas and Bacillus. Additionally, we found two poten-tial novel species. The result shows that the bacterial population diversity is abundant in B. fangiana rhizosphere soil.