青海湖地区狗獾分类地位和狗獾属进化历史探讨

doi:10.17520/biods.2016003

摘要/Abstract

摘要：

本研究选择线粒体细胞色素b (cytochrome b, Cyt b)和控制区(control region, CR)片段作为分子标记, 探讨了青海湖地区狗獾(Meles sp.)的系统发育地位和狗獾属分歧时间。研究结果支持目前将狗獾属分为4个种的结论。Cyt b和CR片段序列拼接后总长1,652 bp, 23条序列共定义了21个单倍型。研究结果表明欧亚大陆狗獾分为东西两个支系, 每个支系进一步分为两个种: 东部支系包括亚洲狗獾(M. leucurus)和日本狗獾(M. anakuma); 西部支系包括欧洲狗獾(M. meles)和西南亚狗獾(M. canescens)。贝叶斯树和单倍型网络关系图都支持青海湖地区狗獾属于亚洲狗獾。分歧时间的估算结果与古生物学证据相符, 东部支系和西部支系在2.24 Ma左右产生分歧, 西南亚狗獾在1.27 Ma左右从欧洲狗獾分出, 而日本狗獾和亚洲狗獾的分化时间为0.99 Ma左右。

关键词: 狗獾, 青海湖地区, 细胞色素b, 控制区, 系统发育关系

Abstract

To investigate the phylogenetic status of badgers (Meles sp.) in the Qinghai Lake area and to estimate the divergence time within Eurasian badgers, the cytochrome b gene and the partial control region (CR) of mitochondrial DNA were examined as genetic markers. Our results indicated that Meles is divided into four species. Twenty one haplotypes were identified in 23 combined sequences (1,652 bp) of Cyt b and CR sequences. Results showed that Eurasian badgers were clearly divided into two major lineages: one of Western lineage, composed of badgers from Europe and Southwest Asia, and the other of Eastern lineage, composed of badgers from North and East Asia (including Japan). Furthermore, the Bayesian tree and the haplotype network indicated that the badgers in the Qinghai Lake area are Asian badgers (M. leucurus). The estimated divergence time was consistent with palaeontological evidence. The first split between the Western lineage and Eastern lineage occurred approximately 2.24 Ma. After the separation, the Southwest Asia clade split from Europe approximately 1.27 Ma and the Japan clade split from continental Asia approximately 0.99 Ma.

Key words: badger, Meles, Qinghai Lake area, cytochrome b, control region, phylogenetic relationship

罗晓, 李峰, 陈静, 蒋志刚 (2016) 青海湖地区狗獾分类地位和狗獾属进化历史探讨. 生物多样性, 24, 694-700. DOI: 10.17520/biods.2016003.

Xiao Luo, Feng Li, Jing Chen, Zhigang Jiang (2016) The taxonomic status of badgers in the Qinghai Lake area and evolutionary history of Meles. Biodiversity Science, 24, 694-700. DOI: 10.17520/biods.2016003.

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链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2016003

https://www.biodiversity-science.net/CN/Y2016/V24/I6/694

图/表 5

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单倍型 Haplotype	样本代码 Sample code	样本编号 Sample no.	采样点 Sampling locality	序列号 Accession no.
单倍型 Haplotype	样本代码 Sample code	样本编号 Sample no.	采样点 Sampling locality	Cyt b	Control region
H1	QH1	B1	中国青海 Qinghai, China	KU361236	KU361238
H2	QH2	B2	中国青海 Qinghai, China	KU361237	KU361239
H2	QH3	B3	中国青海 Qinghai, China	KU361237	KU361239
H3	QH4	B4	中国青海 Qinghai, China	-	KU361239
H4	QH5	B5	中国青海 Qinghai, China	-	KU361240
H4	QH6	B6	中国青海 Qinghai, China	-	KU361240
H5	China	-	中国黑龙江 Heilongjiang, China	KU052604	KU052604
H6	Sweden	-	瑞典 Sweden	AM711900	AM711900
H7	Mongolia	45Mo	蒙古 Mongolia	HQ711950	AJ563694
H8	Greece	80Cr	希腊克里特岛 Crete Island, Greece	HQ711947	GU247573
H9	Israel1	49Is	以色列 Israel	HQ711946	AJ563686
H10	Israel2	48Is	以色列 Israel	HQ711945	AJ563685
H11	Spain	35Sp	西班牙 Spain	HQ711943	AJ563676
H12	Russia1	ZIS33	俄罗斯外贝加尔地区 Transbaikalia, Russia	AB049807	AB538995
H13	Russia2	ZIS36	俄罗斯列宁格勒州 Leningrad Province, Russia	AB049808	AB538997
H14	Russia3	ZIS35	俄罗斯列宁格勒州 Leningrad Province, Russia	AB049809	AB538998
H15	Japan1	K1	日本九州大分县 Oita, Kyushu, Japan	AB049806	AB538971
H16	Japan2	K6	日本九州大分县 Oita, Kyushu, Japan	AB049800	AB538971
H17	Japan3	K7	日本九州大分县 Oita, Kyushu, Japan	AB049802	AB538971
H18	Japan4	K8	日本九州福冈县 Fukuoka, Kyushu, Japan	AB049799	AB538971
H19	Japan5	YMG1	日本本州山口县 Yamaguchi, Honshu, Japan	AB049795	AB538983
H20	Japan6	MR1	日本九州岩手县 Iwate, Kyushu, Japan	AB049791	AB538977
H21	Japan7	KPM-NF1002945	日本本州神奈川县 Kanagawa, Honshu, Japan	AB291075	AB291075
	Arctonyx collaris	YP6001		HM106329	HM106329

单倍型 Haplotype	样本代码 Sample code	样本编号 Sample no.	采样点 Sampling locality	序列号 Accession no.
单倍型 Haplotype	样本代码 Sample code	样本编号 Sample no.	采样点 Sampling locality	Cyt b	Control region
H1	QH1	B1	中国青海 Qinghai, China	KU361236	KU361238
H2	QH2	B2	中国青海 Qinghai, China	KU361237	KU361239
H2	QH3	B3	中国青海 Qinghai, China	KU361237	KU361239
H3	QH4	B4	中国青海 Qinghai, China	-	KU361239
H4	QH5	B5	中国青海 Qinghai, China	-	KU361240
H4	QH6	B6	中国青海 Qinghai, China	-	KU361240
H5	China	-	中国黑龙江 Heilongjiang, China	KU052604	KU052604
H6	Sweden	-	瑞典 Sweden	AM711900	AM711900
H7	Mongolia	45Mo	蒙古 Mongolia	HQ711950	AJ563694
H8	Greece	80Cr	希腊克里特岛 Crete Island, Greece	HQ711947	GU247573
H9	Israel1	49Is	以色列 Israel	HQ711946	AJ563686
H10	Israel2	48Is	以色列 Israel	HQ711945	AJ563685
H11	Spain	35Sp	西班牙 Spain	HQ711943	AJ563676
H12	Russia1	ZIS33	俄罗斯外贝加尔地区 Transbaikalia, Russia	AB049807	AB538995
H13	Russia2	ZIS36	俄罗斯列宁格勒州 Leningrad Province, Russia	AB049808	AB538997
H14	Russia3	ZIS35	俄罗斯列宁格勒州 Leningrad Province, Russia	AB049809	AB538998
H15	Japan1	K1	日本九州大分县 Oita, Kyushu, Japan	AB049806	AB538971
H16	Japan2	K6	日本九州大分县 Oita, Kyushu, Japan	AB049800	AB538971
H17	Japan3	K7	日本九州大分县 Oita, Kyushu, Japan	AB049802	AB538971
H18	Japan4	K8	日本九州福冈县 Fukuoka, Kyushu, Japan	AB049799	AB538971
H19	Japan5	YMG1	日本本州山口县 Yamaguchi, Honshu, Japan	AB049795	AB538983
H20	Japan6	MR1	日本九州岩手县 Iwate, Kyushu, Japan	AB049791	AB538977
H21	Japan7	KPM-NF1002945	日本本州神奈川县 Kanagawa, Honshu, Japan	AB291075	AB291075
	Arctonyx collaris	YP6001		HM106329	HM106329

	西南亚狗獾 M. canescens	欧洲狗獾 M. meles	日本狗獾 M. anakuma	亚洲狗獾 M. leucurus
欧洲狗獾 M. meles	0.034 ± 0.007
日本狗獾 M. anakuma	0.042 ± 0.009	0.043 ± 0.008
亚洲狗獾 M. leucurus	0.040 ± 0.008	0.041 ± 0.008	0.021 ± 0.005
青海湖地区狗獾 Badger in the Qinghai Lake area	0.038 ± 0.008	0.041 ± 0.008	0.020 ± 0.006	0.017 ± 0.004

	西南亚狗獾 M. canescens	欧洲狗獾 M. meles	日本狗獾 M. anakuma	亚洲狗獾 M. leucurus
欧洲狗獾 M. meles	0.034 ± 0.007
日本狗獾 M. anakuma	0.042 ± 0.009	0.043 ± 0.008
亚洲狗獾 M. leucurus	0.040 ± 0.008	0.041 ± 0.008	0.021 ± 0.005
青海湖地区狗獾 Badger in the Qinghai Lake area	0.038 ± 0.008	0.041 ± 0.008	0.020 ± 0.006	0.017 ± 0.004