生物多样性 ›› 2012, Vol. 20 ›› Issue (6): 703-709.  DOI: 10.3724/SP.J.1003.2012.07066

• 研究报告 • 上一篇    下一篇

广西3科植物内生拟盘多毛孢多样性

李曼1, 陈唯王1, 韦继光1,*(), 宋利沙1, 黄松殿2, 罗基同3, 潘秀湖4   

  1. 1 广西大学农学院, 南宁 530005
    2 广西南宁良凤江国家森林公园, 南宁 530031
    3 广西壮族自治区森林病虫害防治站, 南宁 530022
    4 广西高峰林场, 南宁 530001
  • 收稿日期:2012-03-01 接受日期:2012-05-10 出版日期:2012-11-20 发布日期:2013-01-04
  • 通讯作者: 韦继光
  • 作者简介:* E-mail: jiguangwei@gxu.edu.cn
  • 基金资助:
    国家自然科学基金(30970022);广西自然科学基金(2011GXNSFA018078)

Diversity of endophytic Pestalotiopsis associated with three plant families in Guangxi

Man Li1, Weiwang Chen1, Jiguang Wei1,*(), Lisha Song1, Songdian Huang2, Jitong Luo3, Xiuhu Pan4   

  1. 1 College of Agriculture, Guangxi University, Nanning 530005
    2 Nanning Liangfengjiang National Forest Park of Guangxi, Nanning 530031
    3 Forest Pest and Disease Control Station of Guangxi, Nanning 530022
    4 Guangxi Gaofeng Tree Farm, Nanning 530001
  • Received:2012-03-01 Accepted:2012-05-10 Online:2012-11-20 Published:2013-01-04
  • Contact: Jiguang Wei

摘要:

为进一步了解植物内生拟盘多毛孢(Pestalotiopsis)的组成及变化规律, 本研究对广西林科院植物园的壳斗科(10种)、樟科(7种)和棕榈科(5种)植物的内生真菌拟盘多毛孢多样性分四季进行了调查。经分离鉴定得拟盘多毛孢属真菌30种, 其中从壳斗科分离到17种、樟科分离到19种、棕榈科分离到17种, 优势种为小孢拟盘多毛孢(P. microspora)。从壳斗科的饭甑青冈(Cyclobalanopsis fleuryi), 樟科的樟树(Cinnamomum camphora)和棕榈科的香桄榔(Arenga engleri)中分离得到的拟盘多毛孢种数分别为8、12和12种, 这3种植物分别是3科中内生拟盘多毛孢定殖种类最多的代表物种; 其中樟树内生拟盘多毛孢定殖率最高(6.67%)。除棕榈科植物软叶针葵(Phoenix roebelenii)外, 内生拟盘多毛孢在植物枝条中的定殖率均高于叶片, 而且这些植物枝条中内生拟盘多毛孢物种多样性更加丰富。春夏秋冬四季分离得到内生拟盘多毛孢分别为18、16、12和10种; 定殖率分别为3.44%、1.53%、1.10%和1.75%; 物种多样性指数分别为2.5006、2.2902、2.2475和1.9541。本研究表明拟盘多毛孢偏好在某些植物种类中定殖, 并有明显的组织选择偏向性, 且定殖率有季节差异性。

关键词: 内生真菌, Pestalotiopsis, 物种多样性, Fagaceae, Lauraceae, Palmae

Abstract

To understand diversity and successional changes of endophytic Pestalotiopsis in plant communities, we completed surveys of endophytic Pestalotiopsis in plants belonging to the Fagaceae (10 species), Lauraceae (7 species), and Palmae (5 species) in the Botanical Garden of Guangxi Academy of Foresty Sciences. We identified 30 endophytic Pestalotiopsis species, of which 17, 19, and 17 endophytic Pestalotiopsis species were obtained from Fagaceae, Lauraceae and Palmae, respectively, with Pestalotiopsis microspora as the dominant species. We collected a total of 8, 12 and 12 endophytic Pestalotiopsis species from Cyclobalanopsis fleuryi (Fagaceae), Cinnamomum camphora (Lauraceae), and Arenga engleri (Palmae), respectively. We found the highest colonization frequency (6.67%) of Pestalotiopsis in Cinnamomum camphora. We also found that colonization frequencies of endophytic Pestalotiopsis were higher on twig tissues compared with leaf tissues for most plant species except Phoenix roebelenii. The total number of Pestalotiopsis species varied by season, with 18, 16, 12, and 10 endophytic Pestalotiopsis species collected in spring, summer, autumn, and winter, respectively. Colonization frequencies of endophytic Pestalotiopsis also varied by season with frequencies of 3.44%, 1.53%, 1.10% and 1.75% in spring, summer, autumn, and winter, respectively. This research showed that Pestalotiopsis had a preference for colonization in some plants and certain tissues, and its colonization frequencies fluctuated in different seasons.

Key words: endophytic fungus, Pestalotiopsis, species diversity, Fagaceae, Lauraceae, Palmae