生物多样性

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大山雀两亚种基因指纹图的比较研究

方盛国   

  1. (四川师范大学分子生物学研究所,  成都 610066)
  • 收稿日期:1995-03-17 修回日期:1996-03-13 出版日期:1996-11-20 发布日期:1996-11-20

A comparative study on the DNA 'fingerprints' of Parus major tibetanus and Parus major artatus

Fang Shengguo   

  1. Institute of Molecular Biology ,Sichuan Normal University ,Chengdu 610066
  • Received:1995-03-17 Revised:1996-03-13 Online:1996-11-20 Published:1996-11-20

摘要:

寡核苷酸基因指纹探针LZF-I对大山雀青藏亚种(Parus major tibetanus)和华北亚种(P.m.artatus)随机个体进行了基因指纹图的比较检测,结果如下:(1)P.m.tibetanus个体在2.3 kb处均有一条杂交谱带,而P.m.artatus个体的共有谱带则位于4.8 kb处。不同的共有等位基因,是区别两亚种的遗传标记之一。(2)P.m.tibetanusP.m.artatus随机个体的平均谱带数分别是14.65条和19.30条;个体间基因指纹图的相似系数分别是0.18和0.13;P.m.tibetanusP.m.artatus个体之间基因指纹图的相似系数是0.09。结果表明:P.m.artatus群体内的遗传变异性高于P.m.tibetanus群体,且两亚种之间的变异性高于两亚种内部的变异性。(3)LZF-I探针在P.m.tibetanus随机个体检测中的鉴别几率为2.04×10-12,在P.m.artatus则高达9.8×10-17。表明两亚种的基因指纹图均具有个体特异性,且P.m.artatus群体基因组的多态性较P.m.tibetanus强。
 该结果为开展对大山雀种内分化的基因多样性和近缘种之间的遗传差异性,以及P.m.artatus分类学地位的最终确立的研究,提供了分子遗传学资料。

Abstract

A comparative study was conducted to determine the DNA 'fingerprints' of P.m.tibetanus and P.m.artatus by employing the oligonucleotide probe LZF-I technique.The important findings are as follows: (1) Identical bands were detected at the 2.3 kb in all of the randomly selected individuals of P.m.tibetanus,and at the 4.8 kb of the P.m.artatus samples.This difference in the alleles could be one of the important genetic markers used to classify the two subspecies. (2) The average bands were 14.65 in P.m.tibetanus, and 19.30 in P.m.artatus.The similarity coefficients among the individuals of P.m.tibetanus and P.m.artatsu were 0.18 and 0.13,respectively.The similarity coefficient between the subspecies was 0.09.These results indicated that the individuals of P.m.artatus had greater genetic variation than those of P.m.tibetanus,and the variations between the two subspecies were greater than between the individuals of each subspecies. (3) The probability of the LZF-I probe in identifying the genetic variation among the individuals of P.m.tibetanus was 2.04×10-12 ,while that was 9.8×-17among the individuals of P.m.artatus,suggesting that there existed specificity of fingerprints among all the individuals of the two subspeciesP,and that the polymorphism of the genomic DNA in .m.artatus was greater than that in P.m.tibetanus.
         The results reported in this paper are of importance in studying the genetic diversity and systematic position of the two species.