生物多样性

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单株根系来源的土著大豆慢生根瘤菌多样性

卜常松,张学江, 江木兰, 胡小加   

  1. (中国农业科学院油料作物研究所,  武汉 430062)
  • 收稿日期:1995-09-01 修回日期:1995-12-16 出版日期:1996-11-20 发布日期:1996-11-20

Diversity among the indigenous Bradyrhizobium japonicum strains from nodules on roots of the single soybean plant

Bu Changsong Zhang Xuejiang Jiang Mulan Hu Xiaojia   

  1. Oil Crops Research Institute , Chinese Agriculture Academy of Sciences , Wuhan 430062
  • Received:1995-09-01 Revised:1995-12-16 Online:1996-11-20 Published:1996-11-20

摘要:

本文对由1株大豆根瘤分离、鉴定的12个土著大豆慢生根瘤菌(Bradyrhizobium japonicum)菌株进行了种内多样性分析。在YEM琼脂培养基上观察菌落大小不同(直径≥1.0 mm至≤0.5 mm)的菌株,有形成粘稠而半透明的M型菌落和稀薄而透明的W型菌落。以ELISA鉴定血清类型,分为LL19血清族(包括Sz1-12s)血清组和Sz1-19s血清型及其他血清组)和非LL19血清族(包括Sz1-13s和Sz1-21s两血清型及未知血清型)。以Digoxigenin标记的pMJS12为探针检测菌株染色体DNA的EcoRI消化产物的分子杂交谱,出现称为Ⅰ型的9.6 kb和Ⅱ型的6.4 kb两种类型的单一分子杂交带。HP5890A气相色谱分析细胞脂肪酸,检出8种成分。其中,C14∶1、C18∶1和C14∶0存在于所有供试菌株中,为不同于快生根瘤菌的3种主要成分,以此可将供试菌株分成6组不同菌株。

Abstract

Intraspecific diversity are analysized for 12 indigenous strains isolated from nodules on roots of the single soybean plant and identified as Bradyrhizobium japonicum strains. Mucoid and translucent colony type M as well as watery and transparent colony type W were observed from different colony size (diameter≥1.0 mm to ≤0.5 mm) formed by the strains on YEM agar. Serological types of the strains were identified by ELISA classfied as serocluster LL19 (including Sz1-12s serogroup Sz1-19s serotype and others) and non-serocluster LL19 (including Sz1-13s serotype Sz1-21s serotype and unknown). The chromosomal DNA's EcoRI-digested products of the strains were hybridized to the digoxigenin-Labled probe, pMJS12 plasmid, producing two kinds of single DNA hybridization fragment in the strains, sized 9.6 kb and 6.4 kb, respectively. Accordingly the strains were classfied as pattern Ⅰand pattern Ⅱ. Up to eight kinds of fatty acid in the strains were determined by HP5890 A gas-chromatography. Among them, principal component analysis of C14∶1, C18∶1and C14∶0 occured in all B. japonicum strains tested, which was greatly different from the results of fast-growing rhizobia, was used to show that the strains could be divided into six groups.