生物多样性 ›› 2006, Vol. 14 ›› Issue (6): 488-497.  DOI: 10.1360/biodiv.060041

• 论文 • 上一篇    下一篇

珍稀濒危药用植物黄檗野生种群遗传多样性的AFLP分析

闫志峰1, 张本刚1, 张昭1*, 于俊林2   

  1. 1 (中国协和医科大学, 中国医学科学院药用植物研究所, 北京 100094)
    2 (通化师范学院化学系, 吉林通化 134002)
  • 收稿日期:2006-03-01 修回日期:2006-08-30 出版日期:2006-11-20 发布日期:2006-11-20
  • 通讯作者: 张昭

Genetic diversity in wild populations of Phellodendron amurense, a rare and endangered medicinal plant, detected by AFLP

Zhifeng Yan1, Bengang Zhang1, Zhao Zhang1*, Junlin Yu2   

  1. 1 Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100094
    2 Department of Chemistry, Tonghua Normal College, Tonghua, Jilin 134002
  • Received:2006-03-01 Revised:2006-08-30 Online:2006-11-20 Published:2006-11-20
  • Contact: Zhao Zhang

摘要: 黄檗(Phellodendron amurense)是我国珍贵的药用和用材树种, 现已被列为国家二级保护植物。为了研究其遗传多样性和遗传结构, 以制定有针对性的保护策略, 作者采用8对荧光引物, 对来自10个野生种群共计129个黄檗个体进行了AFLP分析。共扩增出1,704条谱带, 其中多态带1,581条。遗传分化系数Gst为0.3420, 即有34.20%的遗传变异来自种群间。黄檗物种水平的遗传多样性高于种群水平: 在物种水平上, 多态位点百分率PPL =92.77 %, 有效等位基因数Ne=1.4636, Nei’s基因多样性指数H=0.2316, Shannon信息指数I=0.4275; 在种群水平上, PPL =54.20%, Ne=1.2487, H=0.1524, I=0.2371。UPGMA聚类结果表明, 黄檗野生种群可分为四类, 并且来自同一地区的黄檗个体有聚为一类的趋势, 说明其遗传变异可能与生境有关。建议在各种群内大量采样,实施迁地保护; 在长白山、完达山、大兴安岭、小兴安岭、千山以及燕山山脉的云蒙山等地区, 均匀布点划分区域进行就地保护, 保护种群完整性, 促进种群自我更新。

AbstractPhellodendron amurense is a rare and endangered medicinal plant in China. We assessed the genetic diversity within and among populations of this species using AFLP. A total of 129 individuals from 10 wild populations were detected by eight pairs of AFLP primers. These primers generated a total of 1,704 bands, of which 1,581 were polymorphic. The results revealed a high level of genetic diversity. At species level, the percentage of polymorphic loci (PPL) was 92.77%, the effective number of alleles per locus (Ne) was 1.4636, Nei’s gene diversity (H) was 0.2316, and Shannon’s information index (I) was 0.4275, respectively. At population level, the estimates of PPL =54.20%, Ne=1.2487, H=0.1524 and I=0.2371. The coefficient of genetic differentiation (Gst) was 0.3420. UPGMA cluster analysis showed that there was a possible correlation between genetic variance and habitat differences. As a conclusion, we propose that conservation of P. amurense should be in their natural habitats, including Mts. Changbaishan, Wandashan, Daxing’anling, and Xiaoxing’anling, Qianshan and Yanshan. At the same time, the preservation of germplasm resources in botanical gardens should be carried out by sampling more individuals from multiple sites.