生物多样性 ›› 2005, Vol. 13 ›› Issue (6): 546-554.  DOI: 10.1360/biodiv.050088

• 论文 • 上一篇    下一篇

岛屿特有种全缘冬青遗传多样性的ISSR分析

冷欣1, 王中生1, 安树青1*, 冯珏1, 刘玉虹1, 王国明2   

  1. 1 (南京大学生命科学学院生态学与进化生物学系, 南京 210093)
    2 (浙江舟山市林业科学研究所, 舟山 316000)
  • 收稿日期:2005-04-11 修回日期:2005-07-18 出版日期:2005-11-20 发布日期:2005-11-20

ISSR analysis of genetic diversity of Ilex integra, an insular endemic plant

Xin Leng1, Zhongsheng Wang1, Shuqing An1*, Jue Feng1, Yuhong Liu1, Guoming Wang2   

  1. 1 Department of Ecology and Evolutionary Biology, School of Life Science, Nanjing University, Nanjing 210093
    2 Zhoushan Forestry Institute of Zhejiang, Zhoushan 316000
  • Received:2005-04-11 Revised:2005-07-18 Online:2005-11-20 Published:2005-11-20

摘要: 全缘冬青(Ilex integra)为岛屿特有植物, 主要分布于浙江舟山群岛, 被列为浙江省珍稀濒危植物。作者运用ISSR分子标记对舟山群岛的6个全缘冬青种群(5个自然种群和1个栽培种群)共57个个体进行了遗传多样性分析。采用9条随机引物扩增出78条清晰谱带, 其中45条为多态性条带, 多态位点百分比(PPL)为57.7%。经POPGENE软件分析, 全缘冬青种群平均多态位点百分比(PPL)为42.1%, Nei’s基因多样度(HE)为0.153, 较其他海岛植物的遗传多样性偏低。5个自然种群遗传分化系数Gst=0.316, AMOVA测度Fst=0.295, Shannon多样性分化系数S=0.304, 遗传分化程度较高。全缘冬青种群间地理距离与遗传距离具有正相关性(r=0.649, P<0.05), 岛屿间地理隔离对种群间遗传分化产生了较为显著的影响。UPGMA聚类分析显示, 朱家尖岛与普陀岛种群亲缘关系较近, 而舟山岛栽培种群是由桃花岛自然种群移植而来。针对全缘冬青种群遗传多样性较低及遗传分化程度较高的现状, 应加强现有自然种群的就地保护, 促进种群自然更新; 建立种质资源库, 收集不同岛屿的种源进行混合繁殖, 促进基因交流。

AbstractIlex integra is an insular endemic and endangered species that is distributed only in East Asia. Here we used inter-simple sequence repeat (ISSR) markers to investigate the genetic structure of six populations (five natural populations and one cultivated population) of the species from Zhoushan Archipelago, China. A total of 78 discernible loci were obtained from 57 individuals of all sampled populations using nine primers. Of these loci, 57.7% were polymorphic (PPL=57.7%). Both the average percentage of polymorphic loci (PPL=42.1%) and Nei’s genetic diversity (HE=0.153), estimated by POPGENE, indicated that I. integra had lower levels of genetic diversity than those reported in many other insular plant species. Our results also showed that populations of I. integra were highly differentiated (Gst=0.316, Fst=0.295,S=0.304). Geographic isolation may be a significant factor to the genetic differentiation among these populations. In addition, a significant correlation was found between genetic distance and geographic distance (r=0.649, P<0.05). UPGMA analysis revealed that Putuo Island population had a close relationship with Zhujiajian Island populations, and the cultivated populations on Zhoushan Island might have originated from Taohua Island. Based on the above findings, we propose that conservation management of I. integra should include protection of natural habitats occupied by all five wild populations and preservation of germplasm in bo-tanical gardens from multiple seed sources.